Absorbance at Various pH Levels
The accompanying table represents the results of an experiment where the effects of pH
buffers on an enzyme found in saliva (amylase) were studied. A spectrophotometer set
at 500nm was used to measure absorbance at the various pH levels every 20 sec for 2
min. The higher absorbance values would indicate greater enzyme activity. All
experiments were conducted at the same temperature.
Which statement correctly identifies the result that the optimum pH for amylase
function is 7?
A) The pH with the lowest absorbance values would indicate the optimum pH for
amylase since this pH does not affect the structure or function of the protein.
B) The pH with the highest absorbance values would indicate the optimum pH for
amylase since this pH does not affect the structure or function of the protein.
C) At pH 9, the enzyme is denatured and will lose its function, but not its structure.
D) At pH 4, the structure of the enzyme will be altered, and the enzyme would not be
able to catalyze the reaction.
Select the choice that correctly associates the organ with its embryonic sources.
A) anterior pituitary gland mesoderm and endoderm
B) thyroid gland mesoderm and ectoderm
C) adrenal gland ectoderm and mesoderm