You are dissecting a “mystery animal” in biology lab and discover that it has a series of
closed blood vessels, a pair of lungs, two atria, and a single ventricle. What animal are
you dissecting?
A) fish
B) frog
C) crocodile
D) bird
After reading the paragraphs below, answer the questions that follow.
Cystic fibrosis is an autosomal recessive genetic disorder that most severely affects the
lungs and respiratory tract. Cystic fibrosis is caused by a mutation in the gene for the
protein cystic fibrosis transmembrane regulator (CFTR), which regulates the movement
of chloride and sodium ions across epithelial cell membranes. When the CFTR gene is
mutated in cystic fibrosis, the defective CFTR protein cannot transport ions properly,
which results in too little water drawn across the membrane and the buildup of thick
and sticky (viscous) mucus, especially in the airways. This leads to difficulty of
breathing and frequent lung infections.
Research has shown that some of the increased thickness and viscosity of the mucus in
the respiratory tract is due to the presence of human DNA. If DNA is present in high
concentrations in solution, the DNA molecules can get entangled and lead to a thick,
viscous solution. To combat this, pharmaceutical companies have developed
recombinant forms of the human deoxyribonuclease I (DNase I) protein. DNase I, after
being translated and modified with sugar groups, is normally produced in human cells
and possibly plays a role in DNA breakdown during apoptosis (programmed cell death).
If you wanted to produce a recombinant form of DNase I, what cell type should you
use?
A) E. coli