In E. coli, to repair a thymine dimer by nucleotide excision repair, in which order do the
necessary enzymes act?
A) nuclease, DNA polymerase III, RNA primase
B) helicase, DNA polymerase I, DNA ligase
C) DNA ligase, nuclease, helicase
D) nuclease, DNA polymerase I, DNA ligase
All animals with eyes or eyespots that have been studied so far share a gene in
common. When mutated, the gene Pax–6 causes lack of eyes in fruit flies, tiny eyes in
mice, and missing irises (and other eye parts) in humans. The sequence of Pax-6 in
humans and mice is identical. There are so few sequence differences with fruit fly
Pax-6 that the human/mouse version can cause eye formation in eyeless fruit flies, even
though vertebrates and invertebrates last shared a common ancestor more than five
hundred million years ago.
Pax-6 usually causes the production of a type of light-receptor pigment. In vertebrate
eyes, though, a different gene (the rh gene family) is responsible for the light-receptor
pigments of the retina. The rh gene, like Pax-6, is ancient. In the marine ragworm, for
example, the rh gene causes production of c-opsin, which helps regulate the worm’s
biological clock. Which of the following most likely accounts for vertebrate vision?
A) The Pax-6 gene mutated to become the rh gene among early mammals.
B) During vertebrate evolution, the rh gene for biological clock opsin was co-opted as a
gene for visual receptor pigments.