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DNA replication in vivo requires a primer with a free 3" end. What molecular species
provides this 3" end, and how is it provided?
Describe the positive control exerted by the catabolite activating protein (CAP). Include
a description of catabolite repression.
Why is mitochondrial DNA often used in construction of phylogenetic trees?
How was it determined that X-rays are mutagenic?
Describe a difference between the RNA polymerases of eukaryotes and prokaryotes.
State a significant difference between X-linked and sex-influenced inheritance.
What is recombinant DNA technology? What would you list as safety issues associated
with recombinant DNA technology?
What is another term for a biological catalyst?
In what way can 5"-azacytidine influence transcription?
Explain what is meant by the term heteroduplex in the context of bacterial
transformation.
Explain the composition and use of minimal medium in the study of bacterial genetics.
What is the specific application of reverse transcriptase in the preparation of cDNA?
Fragile X syndrome is a neurological disorder that is caused by a dominantly inherited
mutant allele on the X chromosome?
In a survey of 240,000 human births, six achondroplastic births were recorded to
parents who were unaffected. Given that this form of dwarfism is caused by a fully
penetrant, dominant, autosomal gene, what is the mutation rate?
What is the general order of genes involved in determining early Drosophila
development?
What is retinoblastoma, and what is its supposed genetic basis?
Describe how different strains of E. coli can reveal different linkage arrangements of
genes in Hfr crosses.
Name one of the botanists who, in 1900, rediscovered the work of Gregor Mendel.
In the formation of nucleosomes, one histone class, H1, is not directly involved, yet it
does associate with DNA to form higher level chromosomal structures. Where does this
histone (H1) associate?
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