virus differently and then pass it back to humans.
C) An influenza virus gains new sequences of DNA from another virus, such as a
herpesvirus; this enables it to be transmitted to a human host.
D) An animal such as a pig is infected with more than one virus, genetic recombination
occurs, the new virus mutates, the virus is passed to a new species such as a bird, and
the virus mutates again and can now be transmitted to humans.
Use the paragraph and accompanying figure to answer the following questions.
Human immunodeficiency virus (HIV) infects cells that have both CD4 and CCR5 cell
surface molecules. The viral nucleic acid molecules are enclosed in a protein capsid,
and the protein capsid is itself contained inside an envelope consisting of a lipid bilayer
membrane and viral glycoproteins. One hypothesis for viral entry into cells is that
binding of HIV membrane glycoproteins to CD4 and CCR5 initiates fusion of the HIV
membrane with the plasma membrane, releasing the viral capsid into the cytoplasm. An
alternative hypothesis is that HIV gains entry into the cell via receptor-mediated
endocytosis, and membrane fusion occurs in the endocytotic vesicle. To test these
alternative hypotheses for HIV entry, researchers labeled the lipids on the HIV
membrane with a red fluorescent dye.
What would be observed by live-cell fluorescence microscopy immediately after HIV
entry if HIV is endocytosed first, and then later fuses with the endocytotic vesicle
membrane?
A) A spot of red fluorescence will be visible on the infected cell’s plasma membrane,
marking the site of membrane fusion and HIV entry.