post-hatching) fish to three concentrations of 17β-estradiol (5, 25, and 100
nanograms/liter) that are within the range of concentrations found in water leaving
sewage treatment plants in different countries. They then examined the proportion of
males and females when the fish exposed at embryo-larval and juvenile stages reached
adulthood. Embryo-larval stage fish that had been exposed to 100 ng/l 17β-estradiol
resulted in adult populations that had substantially more females than males compared
to control groups. Embryo-larval fish that had been exposed to 5 and 25 ng/l of
17β-estradiol did not show a statistically significant shift in the proportion of females.
(Brion, F., C. R. Tyler, X. Palazzi, B. Laillet, J. M. Porcher, J. Garric, and P.
Flammarion. 2004. Impacts of 17β-estradiol, including environmentally relevant
concentrations, on reproduction after exposure during embryo-larval-, juvenile-, and
adult-life stages in zebrafish (Danio rerio). Aquatic Toxicology 68:193-217.)
Refer to the paragraph on the effects of estrogens in the environment. What is the
significance of using the concentrations of 5, 25, and 100 ng/l of 17β-estradiol for the
dose in this experiment?
A) These concentrations are similar to those found in many animals.
B) These concentrations are found in the environment.
C) These concentrations are effective, yet not lethal to the fish.
D) These concentrations are standard in toxicology assays.
Which of the following are directly associated with photosystem I?
A) receiving electrons from the thylakoid membrane electron transport chain
B) generation of molecular oxygen
C) extraction of hydrogen electrons from the splitting of water
D) passing electrons to the cytochrome complex