After reading the paragraph below, answer the questions that follow.
While DNA microarrays can be used to determine whether or not a gene is expressed in
a cell, quantitative polymerase chain reaction (qPCR) can be used to measure the extent
of gene expression in cells and tissues. To perform qPCR, mRNA is extracted from cells
and reverse transcribed to complementary DNA (cDNA). Using fluorescent DNA
molecules that recognize specific gene sequences and DNA polymerase, copies of the
gene sequence of interest are made. After the copies are made, a fluorescent detector
measures the intensity of the fluorescent signal, which is proportional to the number of
mRNA transcripts in the cell and corresponds to the level of gene expression in the cell.
In a muscle cell, the contractile protein myosin is present in larger quantities than the
structural protein actin. Which of the following qPCR results would support this
observation?
A) The fluorescent signal for myosin is higher than that of actin.
B) The fluorescent signal for actin is higher than that of myosin.
C) The fluorescent signals for myosin and actin are equal.
D) The fluorescent signal for myosin is large and is zero for actin.
Plants have specific temperatures (or temperature ranges) that allow for maximum
growth. A process by which heat from ________ is transferred from the sun to leaves
helps give plants these ideal temperatures.
A) convection
B) evaporation
C) conduction