Organisms share many conserved core processes and features, including transcription
and translation using a uniform genetic code. Scientists have used these shared
processes and features in biotechnology. For example, for the process of some
transformations, a plasmid is constructed when a eukaryotic gene of interest is added
with an antibiotic resistant gene such beta-lactamase, which is used for ampicillin
resistance. This plasmid is then inserted into a prokaryotic bacterial cell, such as E-coli,
through a transformation process that leads to the production of the product protein
from the eukaryotic organism. To culture the bacteria and obtain the protein product, the
bacteria must grow.
Select the appropriate condition to determine if the plasmid has entered the E-coli
bacterial cell.
A) Nutrient broth to which no antibiotic has been added.
B) Water to which ampicillin has been added.
C) Nutrient broth to which ampicillin has been added.
D) Nutrient broth to which other resistant bacteria have been added.
The following questions refer to the pedigree chart in the figure below for a family,
some of whose members exhibit the dominant trait, W. Affected individuals are
indicated by a dark square or circle.