involved in cell crawling in protists. Anatomical studies had identified the cytoskeletal
protein actin just below the surface of the cell membrane in several species of protist,
but physiological studies had failed to show a functional link between actin, myosin,
and cell crawling.
In a study by N. Poulsen et al. (Diatom gliding is the result of an actin-myosin motility
system, Cell Motility and the Cytoskeleton 44 (1999):23-22), researchers tested whether
motility in a particular species of diatom involves interactions between actin and
myosin.
Refer to the study by Poulsen et al. and the figure above. The data graphed in the figure
could be an artifact if latrunculin A kills this species of diatoms (that is, that may be
why the cells are not moving). Which of the following would be the best evidence that
latrunculin A is NOT killing the cells?
A) When the toxin was washed off the culture, the cells began to move again.
B) There were still a small percentage of motile cells in the culture treated with the
toxin.
C) Most of the cells in the control were moving, indicating that they were alive.
D) When the toxin was applied to another species of diatom, 25% of them continued to
move.