Organisms share many conserved core processes and features, including transcription
and translation using a uniform genetic code. Scientists have used these shared
processes and features in biotechnology. For example, for the process of some
transformations, a plasmid is constructed when a eukaryotic gene of interest is added
with an antibiotic resistant gene such beta-lactamase, which is used for ampicillin
resistance. This plasmid is then inserted into a prokaryotic bacterial cell, such as E-coli,
through a transformation process that leads to the production of the product protein
from the eukaryotic organism. To culture the bacteria and obtain the protein product, the
bacteria must grow.
Select the appropriate condition to determine if the plasmid has entered the E-coli
bacterial cell.
A) Nutrient broth to which no antibiotic has been added.
B) Water to which ampicillin has been added.
C) Nutrient broth to which ampicillin has been added.
D) Nutrient broth to which other resistant bacteria have been added.
For most terrestrial ecosystems, pyramids composed of species abundances, biomass,
and energy are similar in that they have a broad base and a narrow top. The primary
reason for this pattern is that _____.
A) secondary consumers and top carnivores require less energy than producers
B) at each step, energy is lost from the system
C) biomagnification of toxic materials limits the secondary consumers and top
carnivores