Diabetes mellitus is a metabolic disease that is characterized by the buildup of glucose
in the blood, or hyperglycemia. Diabetes results either from the pancreas not being able
to produce the hormone insulin (type 1 diabetes) or if the body’s cells become resistant
to insulin (type 2 diabetes). Insulin is a protein that binds to receptors on cell surfaces to
allow glucose to enter the cell.
In order to manage the disease, type 1 diabetics require frequent insulin injections. Until
the 1970s, insulin was obtained from processing the pancreases of large mammals such
as cows and pigs; it was then purified for medicinal use. This all changed in the 1970s
with the advent of recombinant DNA technology, which allows scientists to insert genes
from other species into bacterial plasmids and have bacteria produce proteins from
these other species’ genes. In 1978, the gene that codes for human insulin was added to
a bacterial plasmid and bacteria were used to produce human insulin. These bacteria
acted as mini-factories that produced human insulin for type 1 diabetes patients. Today,
the production of human insulin from bacteria is commonplace and is a multibillion
dollar market for pharmaceutical companies.
In order to add the gene for human insulin to a bacterial plasmid, the DNA molecules
have to be “cut” with enzymes called restriction endonucleases and then pasted back
together with enzymes called DNA ligases. Imagine that during this process, the first
five nucleotides of the human insulin gene were accidentally cut out before it was
pasted into the bacterial plasmid. What is the most likely outcome if this plasmid was
added to bacterial cells?
A) Transcription would not take place because the RNA polymerase would not
recognize the promoter.
B) The human insulin protein would be produced as normal.
C) The human insulin protein would not be produced because the start codon is
missing.
D) The human insulin protein would be shorter than normal because it will be missing
one amino acid.
Suppose that diploid cells of the seminiferous tubules encounter errors during the cell
cycle and mitosis. This results in the primary spermatocytes having a 4n number of