Oncologists (medical doctors who treat cancer patients) routinely take biopsies
(samples) of tissue from patients to determine whether the tissue is cancerous or not.
What would be the best experiment to do to determine whether cells from the tissue
sample are cancerous?
A) Count the number of chromosomes in the cells from the tissue sample and compare
to the number of chromosomes in noncancerous cells from the patient.
B) Add cells from the tissue sample to a cell culture dish and compare their growth
against a sample of noncancerous cells from the patient.
C) Measure the amount of DNA in G1 in the cells from the tissue sample and compare it
to the amount of DNA in G2 in noncancerous cells from the patient.
D) Add cells from the tissue sample to a rat to see whether the rat develops cancer or
not.
When cloning a gene, one of the steps is to use restriction enzymes to insert the gene of
interest into a vector. If you separated the empty vector (the vector without the gene of
interest) and the cloned vector (the vector that has the gene of interest added) using
agarose gel electrophoresis, what do you expect to observe on the agarose gel if both
vectors are loaded at the same position at the top of the gel?
A) The empty vector would migrate farther down than the cloned vector.
B) The cloned vector would migrate farther down than the cloned vector.