sewage treatment plants in different countries. They then examined the proportion of
males and females when the fish exposed at embryo-larval and juvenile stages reached
adulthood. Embryo-larval stage fish that had been exposed to 100 ng/l 17β-estradiol
resulted in adult populations that had substantially more females than males compared
to control groups. Embryo-larval fish that had been exposed to 5 and 25 ng/l of
17β-estradiol did not show a statistically significant shift in the proportion of females.
(Brion, F., C. R. Tyler, X. Palazzi, B. Laillet, J. M. Porcher, J. Garric, and P.
Flammarion. 2004. Impacts of 17β-estradiol, including environmentally relevant
concentrations, on reproduction after exposure during embryo-larval-, juvenile-, and
adult-life stages in zebrafish (Danio rerio). Aquatic Toxicology 68:193-217.)
Refer to the paragraph on the effects of estrogens in the environment. You are assigned
to write the report to the Environmental Protection Agency, which needs to decide what
level of 17β-estradiol to permit in sewage output. You do not want to make the level
any lower than necessary, because it requires substantial additional money for the extra
treatment of sewage. Given the data presented above, what level of 17β-estradiol would
you suggest is safe to prevent feminization of fish?
A) 2.5 ng/l
B) 12.5 ng/l
C) 25 ng/l
D) 100 ng/l
Within a normally functioning kidney, blood can be found in _____.
A) the vasa recta
B) Bowman’s capsule
C) the proximal tubule
D) the collecting duct