Quiz Question Pool 5 Spring 2017
Hint: see your textbook Chapter 7.3 for info on phage recombination; Chapter 9 contains info on
cloning. NB: the answers to most questions can be found in your lab manual!
Questions on phage:
1) What is a cistron? (2 points)
• A section of DNA or RNA that codes for specific polypeptide in protein synthesis.
2) a. If 2 different mutant rII phages are able to lyse E. coli K (our lab manual’s shorthand for K12(λ))
when they co-infect it, while neither mutant can lyse it separately, what can you say about the 2
mutations? (2 points)
• They are co-dominant
b. What is the name for this kind of test? (1 point)
3) The 2 phage mutants we are using in lab, rII 29 & rII 31, are NOT able to lyse E. coli K when they
co-infect it. What does this tell you about the 2 mutations? (2 points)
• They are independent
4) If E. coli B is co-infected with rII 29 & rII 31, a small percentage of the progeny phage are able to
lyse E. coli K. Draw a picture that shows what happened. (3 points)
5) What does chloroform do? (2 points)
