Gene 3200 Fall 2016 Exam 5 TFMC
True/False (Answer on scantron. A = True, B = False)
11. F A cDNA library produced from a single tissue of a multicellular organism is expected to contain all of the
coding genes in the organism’s genome.
12. T Compound heterozygotes carry two different mutant alleles at a particular gene locus.
13. F GWAS can identify genomic regions associated with polygenic phenotypes and usually directly identify
causative mutations.
14. F To generate a genetic linkage map, DNA sequence information is required.
15. T A genomic library for an organism can be produced by cutting genomic DNA into fragments, ligating the
fragments into a cloning vector, and inserting each cloned fragment into a separate bacterial cell.
16. T The frequency at which codon variants are used can differ between organisms. This can affect the
efficiency of transgene expression when the gene from one organism is transferred into a different species.
17. F Researchers isolate a mouse mutation that results in liver defects. Other tissues do not exhibit a mutant
phenotype. This is an example of a conditional mutation, since the mutation only causes defects in the liver.
18. F Recessive lethal markers on balancer chromosomes help to prevent crossover events between balancer
chromosomes and normal chromosomes.
19. F The CRISPR/cas system is an adaptive immune system found in eukaryotes that can provide heritable
immunity against viruses.
20. T Acellular fetal DNA can be found in the blood of pregnant women, but this DNA is highly fragmented.
21. T The Tilling technique involves the generation of randomly induced mutations followed by targeted
screening to identify mutations in a gene of interest.
