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Amount and Localization of Acetylated Tubulin in Zebrafish Embryos
Introduction:
The zebra fish, Danio rerio, is a popular model organism used for in vivo experiments
and studies in cell biology. One main subject of study in the zebrafish is from that of GFAP, glial
brillary acidic protein. This protein structure is found within the glial cells, both in the brain,
the nervous system, and the eyes of the zebrafish. The GFAP is important for proper brain
function and provides metabolic support to neurons. A series of steps must be followed in order
to properly study these proteins. These proteins must be isolated and various concentrations of
these isolations obtained in order to yield a spectroscopy standard curve comprised of
absorbance vs protein concentration using a method known as Bradford Assay. This method
uses a blue dye called Coomassie Brilliant Blue to determine the absorbance of the protein and
the concentration present. Addition of antibodies into the zebrafish causes these antibodies to
bind to specific proteins in order to identify which specific proteins are characterized by this
interaction. There are two methods to detect the presence of antibodies. These methods are
enzymatic amplification and fluorescent-labeling. Enzymatic amplification uses substrates that
bind to the enzymes a,ached to the antibody creating a pla-orm for visible gathered output
through the form of visible light or a visible precipitate. The .uorescent-labeling technique uses
