Rolando Gomez
Introduction
Researchers have recently detailed that mass extinctions of marine creatures may before long be
happening at alarmingly fast rates than already anticipated due to contamination, rising water
temperatures and loss of environment. Numerous land species also confront a comparable
destiny for the same reasons. But maybe the greatest premonition threat of all confronting people
is the misfortune of the worldwide honeybee population. The result of a passing on bee
population impacts man at the most noteworthy levels on our food chain, posing a massively
grave danger to human survival. Not to mention, that within the last half decade alone 30% of
the national bee population has vanished and about a third of all bee colonies in the U.S. have
died. Although, the rate of bee eradication is developing each year, 42% more last year than the
year before, even at the current annual rate the evaluated financial loss is a colossal 30 billion
dollars a year. (Hagopian, 2014).
Habitat loss is the major risk to bee diversity, while intrusive species, developing
illnesses, pesticide use, and climate change too have the potential to affect bee populations. We
recommend that future preservation techniques need to prioritize, like limiting environment lost,
making rural living spaces bee-friendly, assessing the value of DNA barcoding for bee
conservation, and joining some data to get it the potential impact of climate change on current
bee diversity. ( Brown, 2009)
Method
In order to do DNA barcoding flow of steps is needed to get to the final result. The
correct handling of procedures is important to the accuracy of the results. These procedures
follow the in order of: Sample collection and DNA preparation, DNA cleaning and
concentration, PCR, gel electrophoresis and DNA sequencing. The first step is collecting the
specimen on which the DNA sequence is going to be done. The specimen collect were honey
bees.
DNA Extraction and Cleaning
To extract and clean the DNA from the specimen, some tissue was remove from the body
of the specimen. The piece of tissue was place into a ZR BashingBead™ Lysis Tube and rapidly
and efficiently lysed by bead beating without using organic denaturants or proteinases. A 750 µl
BashingBead™ Buffer was added to the tube. The sample was placed in a bead beater and
processed a high speed for 10 to 15 minutes. Once the sample was ready, it was centrifugated in
a microcentrifuge at ≥10,000 x g for 1 minute. Up to 400 µl supernatant to a Zymo-Spin™ III–F
